Reporter
GFP-ASV

Part:BBa_K1639023:Design

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-18)


GFP with an ASV degradation tag with a half-life of ~110 min


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We already designed it in tagged form. Anybody can express it with given sequence directly. It is a subpart of BBa_K1639000. You can take it from this biobrick by digesting BamH1 or just synthesize it in given form

Source

This part is ASV tagged version of jellyfish Aequorea victoria.

References

Andersen J. B., Sternberg C., Poulsen L. K., Bjørn S. P., Givskov M. ve Molin S. 1998. "New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria". Applied and Environmental Microbiology, 64(6), 2240-2246.